Microscopy : Stomata comparison from leaf prints

I made epoxy leaf print of plants from around my home by using 10 minute epoxy on acrylic or glass slides.

Brassica oleracea (Alboglabra group, AKA Kailan)  X600

Note the high density of very small stomata on this leaf as compared to those on the other plants shown below. These stomata are closed because the it came from a vegetable that has been in the fridge for several days.

Portulaca (AKA Purslane. Exact species indeterminate) X600

These stomata appear enormous when compared to those from Kale. These came fresh from my garden so the stomata are in open position.

Petal print from Caesalpinia pulcherrima f. flava (AKA Peacock flower) X600

Even petals have stomata! These stomata are closed as the petal was picked just after sunrise.


"Needle" print from Casuarina Equisetifolia X400

Casuarina trees are a common sight along Singapore's beaches. The have needle-like leaves (modified branchlets called cladodes) which you can break and gingerly stick together again before passing it off to a friend to guess the point of breakage. Although the lateral leaf print does not reveal the transverse structure of ridges that run along the length of the needles, it is evident the stomata are very small with no discernable guard cells in this 400 magnification picture. Due to the deeply recessed ridges, my 60X objective could not be brought to focus on these pit-like stomata.



Microscope : Olympus CHB, 10X eyepiece, 60X and 40X objective (Condenser stopped down about 2/3 way)
Camera : LG Optimus black

Polarized Light Microscopy - Vanillin

If you've eaten anything that was vanilla flavoured, then you know the smell of vanillin.
I purchased a bottle of what appears to be white crystals of synthetic vanillin from a baking supplies store. A small pinch of crystals were wedged under a cover slip and gently heated until they have melted. The solid-liquid melting transition is surprising sharp compared to sugar and yields beautiful fields of crystals.

The initial melt yielded circular crystal patterns due to crystal formation around random seeds

Unpolarized View of vanillin crystals

Vanillin crystals through crossed polarizers

Upon gingerly reheating and partially remelting 3/4 of the vanillin on the slide, the melt reform by using previously formed crystal boundaries from unmelted crystals from the previous melt.

Vanillin crystals on right are remelted crystals re-forming against boundaries from unmelted circular seed from initial melt.

Ripple-like formations. I speculate some mechanism akin to non-linearities in 2-d laminar flows at work here.

Remelt crystal fields (Above) recrystalize in a more uniform manner than crystal fields seeded by random seed cooling out of the melt(Below).

I spent hours examining the crystals. The variations in the landscape are myrid and mesmerizing. I was listening to Smetana's Moldau and it was a fittingly entrancing accompaniment.

Gaze upon this wind-swept field of pastel blue and pink wheat rendered à la Picaso

A Rorschach inkblot test! What do you see? I choose to percieve a blue rose.

The Vanillin Neptune is pink and its dark side is blue-green. It spins calmly in space while electrical storms flash in its atmosphere.

Microscpe : Olympus CHB x10(0.25) objective with x10 eyepiece
Camera : LG Optimus Black (1.75 stops underexposure with "Vivid" color option)

Polarized Light Microscopy

A student of mine tore apart his old digital dictionary that he was going to toss in the trash. We had a nice time discussing its construction and he gave me its LCD screen from which I tore off a polarizing film. I cut the film into discs and added them to the light path of my Olympus CHB microscope. One went under the eyepiece turret while the other under the condenser.

These are the results:
Citric Acid Crystals at 100 times magnification (Camera : LG Optimus Black)

Small Citric Acid Crystal at 400 times magnification  

A technical treatment of this is available here:
http://www.microscopyu.com/articles/polarized/polarizedintro.html